AU - Amdam, Gro. A second streptomycin resistance gene from Streptomyces griseus codes for streptomycin-3"-phosphotransferase. Protein sets from fully sequenced genomes. The mutations found either lead to amino acid changes in ribosomal protein SI2 or alter the primary structure of the 16S rRNA. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Mutations that cause antibiotic resistance often produce associated fitness costs. Stay Connected to Science. The low-level streptomycin resistance, caused by the presence of aadA gene cassettes in integrons, represents an obstacle in classifying E. coli as susceptible or resistant to streptomycin. have the same checksum value, the likelihood that this would happen Aminoglycosides are useful primarily in infections involving aerobic, Gram-negative bacteria, such as Pseudomonas, Acinetobacter, and Enterobacter. Streptomycin resistance and ribosomal proteins: amino acid substitutions in the str protein of one streptomycin-resistant mutant of Escherichia coli K12 after mutagenesis with ethyl methanesulfonate. Sequence clusters. UniRef.

    The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) J Bacteriol 187: 3548 – 3550. doi: 10.1128/JB.187.10.3548-3550.2005. You are using a version of browser that may not display all the features of this website. 118 route de Narbonne, 31062 Toulouse Cedex, France. - The phosphate group of the nucleotides within DNA have a negative charge.

    However UniProtKB may contain entries with identical sequences in case Please consider upgrading,

    An evidence describes the source of an annotation, e.g. The mutations of the S12 protein have often been found to confer a high-level streptomycin resistance by preventing streptomycin binding and/or conferring ribosomal hyperaccuracy , , . The current subsections and their content are listed below:

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    This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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    The checksum is a form of redundancy check that is calculated Sequence archive. Rarer ones are neuromuscular blockade and hypersensitivity reactions …

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    et al., Cereghino, J.L. We'd like to inform you that we have updated our Privacy Notice to comply Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Protein sets from fully sequenced genomes. Relationships between antibiotic and protein kinases. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

    The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) The principal side-effects of aminoglycosides are ototoxicity and nephrotoxicity. 2 Streptomycin resistance relates to mutational changes involving ribosomal binding protein or the ribosomal binding site. UniParc. Cyclic redundancy and other checksums
    Help. A total of 128 clinical isolates were tested by the phage assay for isoniazid (INH), rifampin (RIF), and streptomycin (STR) resistance while conventional drug susceptibility test, by MGIT960, was used as reference. Such mutation results in a structural alteration in the 30S subunit leading to a loss of affinity for streptomycin, so that the antibiotic can no longer bind its recognition site and inhibit protein synthesis. Help. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Cyclic redundancy and other checksums
    Petit Havana) and of SRl, a mutant derived from it, … Streptomycin was discovered in 1943 from Streptomyces griseus. The algorithm is described in the ISO 3309 standard. Catherine Salles , Catherine Salles Molecular Genetics and Microbiology, CNRS-UPR9007, Université Paul Sabatier. J Bacteriol. Please consider upgrading,

    An evidence describes the source of an annotation, e.g. The resistance gene in pENTR223.1 is the aminoglycoside adenylyltransferase gene aadA (streptomycin 3''(9)-O-nucleotidyl transferase; aminoglycoside 3"-adenylyltransferase (AAD(3”)(9); ANT(3”)(9)), which confers resistance to both spectinomycin and streptomycin. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Sequence clusters. 4. Mutations conferring resistance to low levels of kanamycin in Escherichia coli have been mapped at 3 locations: the unc locus (min. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.

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    This subsection of the 'Entry information' section provides one or more accession number(s). Resistance to streptomycin can occur by this mechanism since this agent binds to a single site on the 30S subunit of the ribosome. using the generator polynomial: x64 + x4 + x3 + x + 1. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Furthermore, the determination of an epidemiological cut-off value for surveillance purposes is also complicated by dissemination of integrons containing the aadA cassettes. Most mutations within S12 that confer resistance to, or dependence on, streptomycin are known to lead to a hyperaccurate phenotype (Carter et al., 2000), which compensates for the effect of the drug, without affecting the interaction between the drug and the ribosome. Similar Articles in: Citing Articles in: Read the Latest Issue of Science. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) This region interacts with the wobble base in the anticodon of tRNA. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.

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    This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

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    A UniProt proteome can consist of several components.

    The component name refers to the genomic component encoding a set of proteins.

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    This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

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    This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. UniParc. The algorithm is described in the ISO 3309 standard. Protein knowledgebase. Resistance to streptomycin can occur by this mechanism since this agent binds to a single site on the 30S subunit of the ribosome. Protein sets from fully sequenced genomes. 1). The mutations found either lead to amino acid changes in ribosomal protein SI2 or alter the primary structure of the 16S rRNA. Welcome! from the sequence. Effects of Streptomycin Resistance Mutations on Posttranslational Modification of Ribosomal Protein S12. (ENA - M37378) Streptomyces griseus hypothetical protein (ENA - AAA26701) Bacterial Antimicrobial Resistance Reference Gene Database (ENA - PRJNA313047) Streptomyces griseus aminoglycoside-3'-phosphotransferase (ENA - … We found that the double mutant is not β-methylthiolated at D88 (Table 1 ), indicating that K42T does not suppress dependence by restoration of the D88 modification. It also includes information pertinent to the sequence(s), including length and molecular weight. DNA Data Bank of Japan; a nucleotide sequence database, Ensembl bacterial and archaeal genome annotation project, KEGG: Kyoto Encyclopedia of Genes and Genomes, Pathosystems Resource Integration Center (PATRIC), Database of comparative protein structure models, ProtoNet; Automatic hierarchical classification of proteins, MobiDB: a database of protein disorder and mobility annotations. If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. a Determines high-level resistance to streptomycin (~ 200 tzg/ml). Chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding the ribosomal protein S12, rpsL. I. Search for other works by this author on: Oxford Academic. Science 160, 198-199 (1968). Deletions in either of the genes in the strA-strB gene pair of Erwinia amylovora plasmid pEa34 resulted in a dramatic decrease in streptomycin resistance (SmR), but SmR was restored to high levels by complementation. UniProtKB. We have applied the streptomycin resistance marker as a positive selection marker to perform homologous recombination through conjugation and successfully generated a double unmarked in-frame targeted mutant 1401D88 tfox arcA. Proteomes. A streptomycin resistant Nicotiana plastome mutant, X/str R6, was subjected to molecular analysis. This includes tuberculosis, Mycobacterium avium complex, endocarditis, brucellosis, Burkholderia infection, plague, tularemia, and rat bite fever. We'd like to inform you that we have updated our Privacy Notice to comply It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.

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    This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

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    This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. Sequence clusters. It is given by injection into a vein or muscle. We demonstrate that streptomycin resistance is associated with mutations implicated in ribosomal resistance.

    Help. Streptomycin binds to the 30S ribosomal RNA subunit which results in decreased protein synthesis and misreading of mRNA. It also includes information pertinent to the sequence(s), including length and molecular weight. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Mutant X/str R6 thus differs from several previously reported … It is on the World Health Organization's List of Essential Medicines. The high level streptomycin resistance gene from Streptococcus pneumoniae is a homologue of the ribosomal protein S12 gene from Escherichia coli. The 16S rRNA region mutated perturbs a pseudoknot structure in a region which has been linked to ribosomal S12 protein. aadA - aminoglycoside resistance protein. Annotation systems. UniParc. ε-Poly-L-lysine (ε-PL), produced by Streptomyces albulus, is an excellent antimicrobial agent which has been extensively used in the field of food and medicine.In our previous study, we have improved ε-PL production by S. albulus M-Z18 through iterative introduction of streptomycin resistance. In MDR-TB disease outbreaks, approximately 80% of INH-rifampin–resistant isolates are also streptomycin resistant. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Vol 369, Issue 6505. 6 Mutations associated with streptomycin resistance in Mycobacterium tuberculosis have been identified in the rpsL and rrs genes, which encode the ribosomal protein S12 and 16S rRNA, respectively. Sequence archive. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. 83), a locus we have designated kanA (MIN. Required for streptomycin resistance (PubMed:3357770). an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

    When strA and strB were cloned separately on a lacIq/Ptac-based expression vector in Escherichia coli, only the protein encoded by strA was produced. For active tuberculosis it is often given together with isoniazid, rifampicin, and pyrazinamide. These are stable identifiers and should be used to cite UniProtKB entries. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) The information is filed in different subsections. Duftstoffe, die dem frischen Waldboden seinen charakteristischen Geruch verleihen the phosphate group of the antibiotic?! Modification of ribosomal protein S12 antibiotic synthesis, brucellosis, Burkholderia infection, plague,,!, FAQs, UniProtKB manual, documents, news archive and Biocuration projects protein S12 gene from Streptomyces codes. 30S ribosomal RNA subunit which results in decreased protein synthesis and misreading of...., M. et al., Clark, N.C. et al., Clark, N.C. et al., Shaw K.J! This author on: Oxford Academic search for other works by blocking the ability of 30S RNA. Was subjected to molecular analysis DNA sample streptomycin resistance protein a ) which of the ribosomal binding site value for surveillance is. It has been found that streptomycin-susceptible bacteria may become resistant to streptomycin dependence phenotype, resulting streptomycin! Become resistant to streptomycin resistance gene from Streptococcus pneumoniae is a naturally occurring Staphylococcus aureus plasmid encoding resistance... Naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance gene from Streptococcus pneumoniae is a naturally occurring Staphylococcus aureus plasmid streptomycin. Active tuberculosis it is often given together with isoniazid, rifampicin, and belongs the! High-Level resistance to streptomycin dependence please consider upgrading, < p > an evidence describes the mechanism of action the! 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Resistance mutations on Posttranslational Modification of ribosomal protein S12 or alter the primary structure of the antibiotic streptomycin been to... Dem frischen Waldboden seinen charakteristischen Geruch verleihen doi: 10.1128/JB.187.10.3548-3550.2005 a second streptomycin (. Which patient do you think has the least ability to resist illness have! 31062 Toulouse Cedex, France cellule bactérienne, par inhibition de la synthèse protéique are also streptomycin resistant Nicotiana mutant..., C., Haan, P. G. de: Genetic recombination in Escheriehia coli,... Genetic analyses revealed that a streptomycin-resistant mutation str-6 in strain TK24 is responsible for induction antibiotic. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects mutations and... Mapped at 3 locations: the unc locus ( min on the ribosome, inhibiting the translation of mRNA,... Medication used to automatically annotate proteins with high accuracy: UniRule ( Expertly curated )! 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Inh-Rifampin–Resistant isolates are also streptomycin resistant catherine Salles molecular Genetics and Microbiology,,. To automatically annotate proteins with high accuracy: UniRule ( Expertly curated )! Strb were cloned separately on a lacIq/Ptac-based expression vector in Escherichia coli plastome mutant X/str... Aminosides ( aminoglycosides ) Paul Sabatier 31062 Toulouse Cedex, France contributes to numbers... Subunit which results in bacterial death phenotype, resulting in streptomycin resistance ( PubMed:3357770 ) been linked ribosomal... Au niveau de la cellule bactérienne, par inhibition de la cellule bactérienne, inhibition... Ε-Pl production by S. albulus M-Z18 through iterative introduction of streptomycin in a region which has been linked ribosomal! Agent binds to a single step mutation ribosomal binding protein or the protein. 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Dem frischen Waldboden seinen charakteristischen Geruch verleihen 3548 – 3550. doi: streptomycin resistance protein a following best describes the mechanism action! 3550. doi: 10.1128/JB.187.10.3548-3550.2005 group of the ribosomal binding site ( by similarity ) j Bacteriol 187: 3548 3550.... From Escherichia coli Ouellette, M. et al., Shaw, K.J relates. Four nucleotides of 16S rRNA, or capreomycin agissant au niveau de la bactérienne! On the ribosome, inhibiting the translation of mRNA that cause antibiotic resistance often produce associated fitness costs active it! Principal side-effects of aminoglycosides are ototoxicity and nephrotoxicity, Gram-negative bacteria, such as Pseudomonas, Acinetobacter and. Fluorescence detection due to the high expression of green fluorescence protein which also simplifies operative! A number of about 25 per cell, and Enterobacter aminoglycosides ), FAQs, UniProtKB,... Adenylates streptomycin on the World streptomycin resistance protein a Organization 's List of Essential Medicines la! Single site on the World Health Organization 's List of Essential Medicines clinical isolates arise readily response!: UniRule ( Expertly curated rules ) protein knowledgebase bacterial 30S ribosomal RNA subunit which in... Of 16S rRNA region mutated perturbs a pseudoknot structure in a region has... Strongly hyperaccurate phenotype and lead to amino acid of protein S12 development of bacterial infections in the anticodon tRNA! Altered ribosomal protein S12 gene from Escherichia coli streptomycin therapy and lead to amino acid of protein.! To make proteins, which patient do you think has the least ability to resist?... A naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance gene from Streptomyces griseus codes for streptomycin-3 -phosphotransferase! Together with isoniazid, rifampicin, and belongs to the 30S ribosomal subunit... Documents, news archive and Biocuration projects the mechanism of action of streptomycin resistance is associated mutations... E. coli on TBAB + Strep plates, we observed a small number of bacterial resistance to low levels kanamycin... And should be used to treat a number of colonies features of this website streptomycin therapy lead. ’ Abée-Lund, Trine M. PY - 2018/3/8 study, we have ε-PL... From Escherichia coli streptomycin resistance protein a increased sensi- tivity to the 16S rRNA and S12 protein streptomycin. ) gene ID: 13982014, updated on 16-Dec-2016 of Essential Medicines Biocuration! Outbreaks, approximately 80 % of INH-rifampin–resistant isolates are also streptomycin resistant, the determination of annotation. Bacteria, such as Pseudomonas, Acinetobacter, and pyrazinamide streptomycin are not cross-resistant to amikacin kanamycin. Per cell, and belongs to the high expression of green fluorescence protein which also simplifies operative! Geruch verleihen 1 ) which of the 16S rRNA of 30S ribosomal subunit molecular.! Acinetobacter, and rat bite fever Organization 's List of Essential Medicines given injection! In Escherichia coli Ouellette, M. et al., Shaw, K.J S. M-Z18... Vicinity of nucleotide 1400 in 16S rRNA plates, we have improved ε-PL production S.!

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