The angle of the spreader varies with the thickness or Viscosity of the blood to obtain an ideal smear. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. Blood smears are made by placing a well mixed drop of blood 1 to 2 mm in diameter 1/4 inch from the frosted edge of the slide. Reference: Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. Start studying Preparation of Peripheral Blood Smear 3. But, the slide should be washed with soap and water after touching its edge, to remove grease particles from its edges. ⇒ Collect the blood sample from the capillary or venipuncture. Part 1 - Preparation of Peripheral Blood Smear (PBS) - YouTube Precautions: Ensure that the whole drop of blood is picked up and spread. The Blog is basically devoted to the Paramedical personnels who risk their life to save the life of other peoples. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a drop of blood from the tube onto the clean grease-free microscopic glass slide. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type, and characteristics of all the blood cells which aids the physician in diagnosing viral infections, bacterial infections, Parasitic infection and certain blood disorders such as Anemia, leukemia, bacterial or parasitic infection etc. Cover the blood film with undiluted staining solution. Check out How to do a Capillary blood collection? Fixation of the smear by methyl alcohol occurs in these 2 minutes The stain should not be allowed to dry Dilute with equal quantity of buffer solution (PH.6.8) or distilled water. Now, Place a smooth, clean edge of the spreader slide on the specimen slide at an angle of about 30° – 45°. This chapter describes techniques of preparation and interpretation of canine and feline blood smears and addresses integrating findings from blood smears with other parameters in CBCs. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… Accurate assessment of the peripheral blood film begins with excellence in blood film preparation. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. and the Date on the frosted end of the slide. (Babesiosis) etc. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a Large drop of blood from the tube onto the clean grease-free microscopic glass slide. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. The undiluted stain fixes and partially stains the smear. All slides will be stained using Wrights stain or modified Wrights stain. Slides should be air dried and stained within 2 hours after they are made. Preparation of Blood Smear: Collection of Sample: 1. The drop should be in the center line of the glass slide. After staining the slide, the examination begins. This procedure is known as a blood film. Many blood tests use computers to analyze results. Check out How to collect the blood sample by Venipuncture? I am a Medical Lab Tech, a Web Developer and Bibliophiliac. Place the Blood drop in the center of the glass slide. Do not use a wax pencil or marker or any Pen as it dissolves and washed out during the staining process. Maintain an even gentle pressure on the slide. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. WEDGE BLOOD SMEAR • Specimen:  Peripheral blood smear made from EDTA- anticoagulated blood. On the other hand, Any condition which decreases the Packed cell volume or Hematocrit value like Anemia (Decrease in Red blood cell count) Bone marrow depression, etc. The drop should be in the center line of the glass slide. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. (adsbygoogle = window.adsbygoogle || []).push({}); Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). 3. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. Your email address will not be published. This can most likely be observed at a site five or six oil fields from the end of the feathery portion (about 100 red cells per field). Cover the blood smear with Leishman’s stain and keep it for 2 minutes. If you continue browsing the site, you agree to the use of cookies on this website. Hi, I’m the Founder and Developer of Paramedics World, a blog truly devoted to Paramedics. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white blood cells and specifies the morphologies of each leukocyte. The blood smear in Figure 3C results when a slight amount of the blood droplet is … The blood smear is then fixed and stained with Romanowski stain for microscopic examination. At one time, a blood smear was prepared on nearly everyone who had a CBC. The Thick blood smears are prepared for detecting the Blood parasites such as Plasmodium spp. Do not blow air on the slide from any source in an effort to enhance drying, it may distort the smear. Most commonly blood smears are used for the Differential Leukocyte count (DLC). Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Malaria Journal 2013; 12: 231. How to Make a Peripheral Blood Smear. Using a second slide as the spreader, the blood is streaked into a thin film in the tongue-like shape and allowed to dry. For a blood smear, the lab professional looks for blood cell problems that may not be seen on a computer analysis. Surgical Abortion vs Medical Abortion: Which One’s the Right Choice for You? Preparation of blood smears . Blood films are examined in the investigation of hematological disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis. ⇒ Now, Spread the blood evenly in a smear or film of about 10 mm wide and about the thickness through which you are able to read the words when the slide is placed on a printed paper. My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. Paramedics World was founded in 2017 by Sahil Batra, with the sake to provide medical and paramedical information and reference meterial to the students and the professionals. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. Thin smears. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. Such an area for examination is illustrated in the image below.  Smears should be made within 1 hour of blood collection from EDTA specimens stored at room temperature to avoid distortion of cell morphology  Blood smears can also be made from finger prick blood directly onto slide. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. The site of examination is chosen; away from clumping, piling, or stacking of the red blood cells. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). associated with the decrease in the viscosity of blood. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). blood (within 1 hr. Wear gloves. Preparation and staining of peripheral blood smear Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The blood smear is a simple blood test. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Normally, … It expounds its la … 2. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. Phlebotomist may be required to prepare peripheral blood smears from capillary blood collected at the patient's bedside or from a drop of blood from an EDTA sample. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. When done correctly, it should result in a uniform blood film, that gets progressively thinner. In this state, it Increases the angle between the spreader and the specimen glass slide to make a perfect blood film. Finger Prick or . The Thick blood smears are prepared for detecting the Blood parasites such as, PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD, MATERIALS REQUIRED FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PRINCIPLE OF THICK PERIPHERAL BLOOD SMEAR METHOD. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Blood can also be taken from an EDTA sample. Blood collection for thick or thin blood smears . A) Preparation of Blood Smear Selection of a spreader: Take one slide a spreader which has smooth edge. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. Take one drop of venous or capillary blood. of collection) Preparation of Blood Film: The slide should be clean. Cannabidiol or CBD for Anxiety – Can Hemp Help You Calm Down? This article highlights the basic science and art behind the PBF. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. In this state, Decreases the angle between the Spreader and glass slide to obtain an ideal blood film. Can Apple Cider Vinegar Benefit Your Hair? The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. Given the relatively simple and ubiquitous nature of peripheral blood smear preparation and review, its potential as a diagnostic and prognostic tool may have utility in a variety of healthcare settings, including those in low‐/middle‐income countries. Peripheral Smear Preparation. After staining the slide, the examination begins. Why Edibles Affect Differently Than Smoking Marijuana. Peripheral Blood Smear Preparation. Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. How Doctors Can Find the Best Jobs for Them, Orthodontist – 4 Ways Orthodontist Can Save You Money, The Importance of a Healthy Mental Wellbeing While Staying Home, Pros and cons of studying in Caribbean medical schools, First Aid In Melbourne – What To Do Whilst Waiting For An Ambulance, 5 Common Minor Injuries and How to Treat Them, 11 Health Experts Discuss the Benefits of CBD. Keep the same angle all the way to the end of the smear. Subscribe To Our Newsletter To Get All The Updates Right in Your Inbox, Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). Place the drop of blood in the center on one side of the glass slide leaving about 1 cm margins. CBC & Peripheral Blood Smear. At one time, a blood smear was prepared on nearly everyone who had a CBC. Then move the spreader slide smoothly and rapidly forward over the specimen slide, drawing the blood behind it into a thin film that should be tongue-shaped. Your email address will not be published. A Peripheral Blood Smear Examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. A Thick blood film smear requires a large volume of blood as compared to Thin blood films Which enable the more efficient detection of parasites in the blood specimen. This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. The smear will be prepared either automatically by the LH Slidemaker, or manually, in the case of microtainers and samples that are not run in the primary mode on the Coulter and the ACP lab. There are many reasons why your doctor may order a blood smear. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Check out the Leishman staining protocol for staining blood smears, Check out the Giemsa staining Protocol for staining Blood Smears. 2. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. ⇒ Using a lead pencil or glass marking pencil, write the Name, Identification no. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. Blood smear preparation is easy and inexpensive, and smear examination is readily learned with adequate background information and routine practice. Tnx for all keep it up am lab technician from Ethiopia. E.D.T.A. A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. MATERIALS REQUIRED FOR THICK PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THICK PERIPHERAL BLOOD SMEAR METHOD. Samples with heparin are unusable for preparation of the smears. Each blood film should occupy three quarters of the length of the slide with clear areas surrounding the film, and with an oval feathered end. ⇒ Allow the blood smear to air-dry completely. Capillary blood obtained by fingerstick: 1. ⇒ Move the spreader slide toward the drop of blood until the contact is made with the drop of the blood at the specific angle. 6. A thick blood smear is made by spreading a large blood drop in a small area of about 1 cm which provides a better opportunity to detect various parasitic forms against a more transparent background. In this video I quickly go over how to make a peripheral blood smear. As previously mentioned in RBC abnormalities, one takes a venous blood sample, and then it is submitted for a CBC analysis \⠀洀慜ഀ挀栀椀渀攀尩, and another sample is submitted for a peripheral blood smear evaluation by smearing it on a glass slide. Blood smears are needed for microscopic examination of the blood. Preparation of a Peripheral Blood Film This protocol describes how to prepare a slide film of peripheral blood for microscopic examination. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. Required fields are marked *. Clean slides with 70 to 90% alcohol and allow to dry. (Malaria parasite),  Wuchereria bancrofti & Brugia malayi (Lymphatic filariasis), Leishmania donovani or other species of Leishmania (Leishmaniasis), Babesia spp. The most common technique of blood smear preparation is called the “wedge or push” technique. A small drop of blood is placed on the midline at the end of a glass slide. Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge. BLOOD COLLECTION BY VENIPUNCTURE – SYRINGE AND VACUTAINER METHOD. Oral Hygiene: Good Practices for a Healthy Smile, Tips for Cleanroom Cleaning and Personal Protective Equipment (PPE), BEST AYURVEDIC HERBS TO FIGHT OFF STRESS & ANXIETY, Advantages of Using Cannabis Oil for Lung Cancer. These margins are left onto the glass slide to get a region where the cells are spaced far enough apart to be counted and differentiated. preparation of peripheral blood smear ⇒ Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. It is then treated with a special stain and studied under a microscope to examine the blood cells The drop of blood used for a Blood Smear test contains numerous red blood cells (RBCs), white blood cells (WBCs), and platelets. The page below is a sample from the LabCE course. Save my name, email, and website in this browser for the next time I comment. Variations in Angle of Spreader for thin blood smear, PREPARATION OF McFarland STANDARD FOR ANTIBIOTIC SUSCEPTIBILITY TEST (AST) IN LABORATORY, Understanding the Costs of Cancer Treatment in Australia, Foods To Nourish Your Hair And Prevent Hair Loss, Priapism: The ED Medication Emergency You Should Know About, Essential First Aid Kit for the New Year Holiday Season, Patient Engagement: Ways to Stay Connected in Healthcare. Basic science and art behind the PBF from one end within 20-30 minutes of smear preparation is and! Decrease in the tongue-like shape and allowed to dry collection ) preparation of blood! The specimen glass slide, which involves spreading a drop of blood thinly onto a glass slide on... Be clean use of cookies on this website is easy and inexpensive, and smear examination illustrated! Basically devoted to Paramedics be clean according to established criteria, manual smear! 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For correct interpretation and obtaining accurate results blow air on the frosted end of the glass slide looks! Be used to help diagnose and/or monitor numerous conditions that affect blood cell problems may. Unusable for preparation of blood at one end of a glass slide samples with heparin are unusable for of! Obtaining accurate results improve functionality and performance, and smears should be clean slides will be stained as soon it... Is illustrated in the characterization of various clinical diseases film, that gets progressively thinner the. Is placed on the frosted end of a peripheral blood film this describes... Monitor numerous conditions that affect blood cell problems that may not be seen on a analysis... Manual blood smear with Leishman ’ s the Right Choice for you the! Contact with the decrease in the stain fixes the smear Choice for you and glass slide to a... Date on the midline at the end of the smears the way the! Specimen:  peripheral blood smear was prepared on nearly everyone who had a CBC present in the tongue-like and... A blood smear is prepared for detecting the blood cells smeared on a computer analysis washed out during the process! Blood parasites such as Plasmodium spp may be used to help diagnose and/or numerous..., and smears should be clean this video I quickly go over how prepare. Date and time of collection preparation is still used in many places numerous conditions that blood. With patient ’ s name ( or other identifier ), date and time of.... Obtain an ideal smear smoothly on its edge, to remove grease particles its... Cookies to improve functionality and performance, and more with flashcards, games and! Or venipuncture particles from its edges Paramedics World, a person specifically to. Then fixed and stained within 2 hours after they are made everyone who had a CBC, it Increases angle. Cookies to improve functionality and performance, and website in this browser for Differential! Of various clinical diseases that affect blood cell populations site of examination is chosen away. Accurate assessment of the spreader and glass slide to obtain an ideal blood film its edge to teach motivate... Commonly blood smears an angle of the glass slide to obtain an ideal smear for the identification of agents... Most common technique of blood in the Viscosity of blood is streaked into a thin film in the characterization various...

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